The Blur frontman's three-minute piece '2LO Calling' was played out by BBC Radio 2's Simon Mayo from London's Science Museum at 5.33pm GMT yesterday (November 14).
Listen to the '2LO Calling' broadcast below:
The event, which brought together more than 55 BBC radio stations, is estimated to have been heard by up to 80 million listeners.
The "snapshot of the airwaves" featured iconic sounds from BBC radio as well as messages from listeners around the world.
'2LO Calling' also includes the chimes of Big Ben, Morse code and philosopher Bertrand Russell's quote: "Love is wise, hatred is foolish."
"There is a special musicality to some of the vocal messages, which I tried to preserve," Albarn told BBC News.
"Added to that, I got to do what I've always wanted - to play along with the pips."
The first transmission from what was then called the British Broadcasting Company - and later renamed the British Broadcasting Corporation - went ahead on November 14, 1922. The BBC had only been formed the previous month, on October 18.
The broadcast came from the transmitter 2LO, located at Marconi House in London, and opened with the now famous words: "This is 2LO calling."