The agreement brings hundreds of episodes of major American shows to LoveFilm's digital streaming service, including the US version of The Office and the multiple-award-winning comedy 30 Rock, starring Tina Fey and Alec Baldwin.
LoveFilm streaming members can now recap on Knight Rider, the 1980s show conceived by heavyweight American TV producer Glen A Larson as "the Lone Ranger with a car".
Knight Rider starred a pre-Baywatch era David Hasselhoff as Michael Knight, a police detective rescued by billionaire Wilton Knight after being shot in the face, and turned into a field agent in a vigilante public justice programme.
Alongside Hasselhoff and his leather jackets, the show was also famous for the Knight Industries Two Thousand (KITT), a heavily modified 1982 Pontiac Trans Am car with lots of gadgets and a disapproving electronic voice.
LoveFilm's NBCUniversal deal also covers hundreds of episodes of other classic US shows, along with more recent TV series.
It follows similar deals agreed by LoveFilm with ABC, the BBC and ITV, as the firm continues its battle with big rival Netflix.
"This deal with NBCUniversal strengthens our exceptional and unrivalled on-demand TV offer," said LoveFilm managing director Jim Buckle.
"Our content, unbeatable value for money and the instant nature of our service make us the first choice for people who want the best possible entertainment experience."
NBCUniversal International TV Distribution president Belinda Menendez added: "We're excited to announce this partnership with LoveFilm and proud of the NBCUniversal titles represented in this agreement.
"LoveFilm customers will now have access to the classic NBCU series they know and love, as well as the opportunity to discover our other high-calibre programming."