Now, they're combining forces as members of a striptease troupe in the eye-watering comedy drama Magic Mike directed by Steven Soderbergh.
Digital Spy caught up with the dynamic duo to find out their position on nudity, grinding masterclasses and getting personal with pythons.
McConaughey said of his reptilian co-star: "He s**t on me. It was four days before shooting. We wanted to get a photo and we thought it was a grand idea that any photo of Dallas would have of himself would be with a python."
Tatum added: "I didn't know what was going on. I didn't know what the picture was for. We were down in California in this condo and we had taken a picture downstairs.
"The next thing I know there's a snake wrangler on set. I walked down and Matthew's got this snake wrapped around him and he's pointing at the photographer. I was like, 'I don't know what this is for but it's awesome!'"
Magic Mike is showing in UK cinemas now.