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The actress told Moviefone that playing Elizabeth Swann in the first three instalments of the Disney franchise was enough.
Johnny Depp has already agreed to a fourth outing as Captain Jack Sparrow and will apparently pick up £32 million for the part.
While Russell Brand has also been linked to the next project, Knightley said: "It was a completely fantastic experience, and it was an amazingly large portion of my life, but I don't think I need to go there again.
"I think that it's done. I'm very excited to see the next one. And [Johnny Depp] is so wonderful in that character. I think it'll be wonderful."
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