The teen vampire flick is up for seven awards, including Best Film, Best Female Performance for Kristen Stewart and Best Breakthrough Male Performance for Robert Pattinson.
Oscar-winning Slumdog Millionaire has taken six nominations, including Best Film and Best Breakthrough Male and Female Performances for Dev Patel and Freida Pinto.
Other films up for gongs at the awards in Los Angeles, California, include The Dark Knight, High School Musical 3: Senior Year and Iron Man.
The host of this year's ceremony has already been revealed as Saturday Night Live comic Andy Samberg, while Eminem has been booked to perform at the show.
> Click here for a full list of MTV Movie Award nominations