The Twilight star received 38% of the vote to beat competition from Christopher Lee and Wesley Snipes in the poll of more than 3,000 cinema-goers.
"People love to be scared witless, particularly at this time of year," said Pearl & Dean chief executive Kathryn Jacob.
"And Edward Cullen being voted firm favourite movie vampire is testament to the fact that people still enjoy a good bloodsucker."
Pattinson, 23, rose to stardom as vampire Edward Cullen in the movie based on the novel by Stephenie Meyer. Sequel New Moon arrives in cinemas next month.
The top ten favourite movie vampires were:
1. Edward Cullen (Robert Pattinson, Twilight)
2. Dracula (Christopher Lee, Hammer Horror films)
3. Blade (Wesley Snipes, Blade)
4. Lestat (Tom Cruise, Interview With The Vampire)
5. Dracula (Gary Oldman, Bram Stoker's Dracula)
6. Selene (Kate Beckinsale, Underworld)
7. David (Kiefer Sutherland, The Lost Boys)
8. Santanico Pandermonium (Salma Hayek, From Dusk Till Dawn)
9. Graf Orlok (Max Schreck, Nosferatu)
10. Dracula (Bela Lugosi, Dracula)