The pair will play against each other in the Warner Bros gospel choir movie, reports Variety.
Bandslam and Camp writer/director Todd Graff is helming the movie. Graff also wrote the script.
The film centres on two strong women who butt heads when they are forced to partner to save a small town gospel choir from folding due to budget cuts.
Latifah will play the mother of two teens who struggles to run the choir after the director dies.
Parton will portray the director's widow, who assumed that she would run the choir after his passing.