The animated movie, which sees Owen Wilson's Lightning McQueen take part in a World Grand Prix, will climax with a race through the streets of London.
The Queen will appear in vehicular form to preside over the finish line after receiving an invite from Sir Miles Axelrod (Eddie Izzard). A character based on Prince William, called Prince Wheeliam, will also appear to cheer on F1 driver Lewis Hamilton's cars character and Nigel Gearsley.
The Brit-themed Cars 2 cast also includes double-decker Topper Deckington III, Buckingham Palace guard Sgt Highgear and black cab Chauncy Fares.
Sir Michael Caine, Jason Isaacs and Emily Mortimer will also voice characters in the 3D movie.
Cars 2 opens in the US on June 24 and July 22 in the UK.
Take a look through our gallery of the Cars 2 Brit characters below: