The actor, best known for the superhero series No Ordinary Family, will take on the role of villain Mr Harter in the film about the television comedy act, according to The Hollywood Reporter.
He will join Will Sasso, Sean Hayes and Chris Diamontopoulos who will play the three leads Curly, Larry and Moe.
Veteran comedian Larry David and Glee star Jane Lynch also recently joined the cast. Lynch will play Mother Superior, a nun who attempts to have the Stooges thrown out of an orphanage that she runs.
Shooting on the movie is expected to begin later this month.
Collins recently spoke out about plans to cancel No Ordinary Family, which revolves around a family who find they have extraordinary powers.
He said that the show deserved to be renewed because it had just "hit its stride".