The 30 Rock star will join returning cast members Mena Suvari, Jason Biggs, Seann William Scott, Thomas Ian Nicholson and Alyson Hannigan, Variety reports.
American Reunion will see the characters meet again at East Great Falls for their high school reunion.
Bowden is to play Chris Klein's girlfriend in the follow-up, which takes place ten years after the first film.
American Reunion will be the first time that several of the original film's cast have worked together since American Pie 2 in 2001.
Executive producer Craig Perry said last year that there is demand for a sequel to American Wedding because the characters are part of the "cultural lexicon".
Bowden will next be seen playing a young actress in The Last Film Festival which sees the late Dennis Hopper in one of his last roles.
Watch the trailer to the original American Pie below: