The kiss came after Pattinson and Kristen Stewart won the 'Best Kiss' prize at the awards show. The pair have now won the award for three years running.
"I feel bad 'cause I don't feel like kissing you," the 25-year-old actor told Stewart on receiving the award.
Stewart replied: "I think maybe we should finally go for it."
Pattinson then hesitated, stating that there was someone else in the audience who deserved the kiss more. He then leaped off the stage and sought out Lautner, kissing him to much applause.
Lautner was also nominated for the 'Best Kiss' award for a lip-lock with Stewart.
It was recently reported that Pattinson and Stewart plan what they will do on stage for this particular award every year they have been nominated.
Last month, Cosmopolis actor Pattinson said that he hopes to get married in the future, declaring that he plans to spend old age in a retirement home with his wife.
The Twilight Saga: Breaking Dawn - Part 1 opens in cinemas on November 18.
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> 'Twilight Saga: Eclipse' wins MTV's 'Best Movie' award
> MTV Movie Awards 2011: The winners
Watch Robert Pattinson and Taylor Lautner's MTV Movie Awards kiss below: