The final instalment of the wizarding franchise collected $92m (£57m) in the US upon its release on Friday, beating previous record-holder The Twilight Saga: New Moon's tally of $72m (£44m) in 2009.
Deathly Hallows: Part 2 raked in an additional $75m (£46m) internationally, to bring its global total close to $250m (£154m). This amount included a first-day opening of almost $15m (£9m) in the UK, the biggest single-day takings ever seen in the country.
The latest Harry Potter also set a new record for midnight screenings, as the $43m (£26m) it collected easily surpassed the $30m (£18m) achieved by late-night shows of The Twilight Saga: Eclipse last year.
"What an incredible beginning for the movie that ends it all," Warner domestic distribution president Dan Fellman commented to Variety.
"Everyone at Warner Bros joins me in congratulating the many people behind the Harry Potter films. We know this is just the start of what promises to be an astounding weekend and a magical summer."
Deathly Hallows: Part 2 took more money in 24 hours than four of the previous seven Harry Potter pictures did over their entire opening weekends, The AP adds. The film's success has been attributed to the increased cost of tickets to 3D showings.
Harry Potter and the Deathly Hallows: Part 2 is also tipped to surpass The Dark Knight's opening weekend record of $158m (£97m) domestically.
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Watch the trailer for Harry Potter and the Deathly Hallows: Part 2 below.