The building, situated 95 miles from Glasgow near Lochgilphead in Argyll, will double as 007's ancestral home and be the location for his final showdown with the movie's villains.
Director Sam Mendes will start filming at Duntrune Castle in February 2012, reports the BBC.
Patricia Malcolm, who resides in the castle with her husband Robin, said: "They are coming here at the end of February and are going to film for several days. They are using the castle - just the outside - it's going to be used for the end of the film.
"I think it is meant to be James Bond's ancestral home - he will bring all the villains here.
"I believe [the film is] mostly being filmed in China and Turkey. They want a winter scene, so they are going to bring their own snow."
The location will allegedly see sets constructed then destroyed in an "explosive" ending in which the castle comes under attack from land and sea.
Bond 23, rumoured to be titled Skyfall, will star Daniel Craig, Bérénice Marlohe, Javier Bardem, Ralph Fiennes, Judi Dench, Naomie Harris, Rory Kinnear, Ben Whishaw and Helen McCrory.
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