Deadline confirms that the Dark Knight star has been cast as Adam Frankenstein in writer-director Stuart Beattie's adaptation of the Kevin Grevioux story.
"Mary Shelley's story is about the creation of the first human being," Beattie said following the announcement. "This is the story about that being becoming human."
"This story is all about what it means to be human, and there could be no more achingly human performance than the one Aaron gave in our own Rabbit Hole," Lionsgate co-chief operating officer Joe Drake added. "We are so excited to see what he will bring to this role."
I, Frankenstein follows Frankenstein's monster, who has miraculously survived to the present day thanks to a rare genetic quirk, and who soon becomes embroiled in a clan war.
Lionsgate will distribute the film in the US, while Lakeshore Entertainment will handle the international release.
Eckhart will next be seen opposite Johnny Depp in Bruce Robinson's adaptation of Hunter S. Thompson's The Rum Diary.
I, Frankenstein will begin filming in January 2012, ready for release on February 22, 2013.
> The Rum Diary - teaser poster unveiled
Watch Aaron Eckhart in the trailer for The Rum Diary below: