The animal rights group has called on Crowe to add a warning about animal safety to We Bought a Zoo, following last week's incident where a man freed lions, a bear and other animals from an Ohio preserve before committing suicide.
Police were forced to kill many of the animals to protect the safety of the surrounding communities.
PETA official Lisa Lange has criticised Crowe for not presenting an accurate portrayal of running an animal sanctuary in We Bought a Zoo, which stars Matt Damon as a father who opens his own zoo in the countryside.
Lange said: "We Bought a Zoo conveys the misleading and downright dangerous message that no special knowledge - just a lot of heart - is needed to run a zoo. As the tragedy in Ohio gruesomely illustrates, wild animals aren't Disney characters.
"They have very special needs that all too often aren't met by people who buy them on a whim because they think it would be cool to own a tiger."
A rep for Crowe hasn't responded to PETA's concerns yet.
We Bought a Zoo opens on December 23 in the US and the UK.
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Watch a trailer for We Bought a Zoo below: