The fourth part in the vampire franchise grossed £13.9 million over the three-day weekend period.
Its Friday figure of £6.35 million surpassed the non-3D opening record set by Harry Potter and the Deathly Hallows: Part 1 last year.
Director of film at Entertainment One Alex Hamilton said: "We have the highest three-day opening ever for an American film in the UK.
"I'd like to thank all the fans of Twilight that have given this three-year-old independent distributor a weekend to remember."
The Twilight Saga: Breaking Dawn - Part 1 has also beaten the biggest 2D opening record for 2011, previously set by comedy spinoff The Inbetweeners Movie.
The vampire film has received negative reviews from a number of critics, being described as "an unconsummated opportunity" and "largely soapy and melodramatic".
> 'Twilight': Team Edward vs Team Jacob - Digital Spy staff verdict