The next instalment of the Kristen Stewart and Robert Pattinson vampire movie made more than $5 million in ticket sales, after its record $12 million start last week.
New entries The Inbetweeners Movie and Immortals take second and third places in the list, followed by seasonal animation film Arthur Christmas, which is voiced by Bill Nighy and James McAvoy.
In fifth place is The Ides of March, which made $941,171 in box office takings, followed by Brad Pitt and Jonah Hill's Moneyball with $677,562.
Other films featuring in the top ten most popular movies at the Australian cinema this weekend include Midnight in Paris and Bollywood new entry Desi Boys.
> 'Twilight: Breaking Dawn' retains UK box office lead - top ten in full
> 'Twilight: Breaking Dawn' leads Thanksgiving weekend - US box office top ten
The box office top ten in full (figures in Australian dollars):
1. (1) The Twilight Saga: Breaking Dawn - Part 1 - $5,020,312
2. (-) The Inbetweeners Movie - $2,855,521
3. (-) Immortals - $1,794,038
4. (-) Arthur Christmas - $1,370,626
5. (-) The Ides of March - $941,171
6. (2) Moneyball - $677,562
7. (3) In Time - $463,531
8. (4) Midnight in Paris - $349,364
9. (6) The Debt - $143,546
10. (-) Desi Boyz - $142,405
Watch Digital Spy interview the cast of Breaking Dawn - Part 1 on the red carpet of the London premiere below: