The 30 Minutes Or Less star made a brief cameo in low-budget picture Camp Hell as a favour to friends who were producing and directing, collecting just $3,000 (£1,900) for his work.
The 28-year-old has now filed a $3m (£1.9m) lawsuit against Lionsgate and Grindstone Entertainment for using his fame to promote the movie without his consent. The figure sought is more than the entire budget of Camp Hell.
Eisenberg's face takes up almost all of the film's DVD cover and his name is written in bold above the title.
"Although Eisenberg appears on screen for less than five minutes, the distributors of Camp Hell are now attempting to fraudulently deceive and mislead the public and reap millions of dollars by capitalising on Eisenberg's newfound fame," legal papers filed in LA state.
"Eisenberg is bringing this lawsuit in order to warn his fans and the public that, contrary to the manner in which Defendants are advertising the film, Eisenberg is not the star of and does not appear in a prominent role in Camp Hell."
The movie, which also stars former Desperate Housewives actress Dana Delany, received a US DVD release in August. It opens in UK cinemas on Friday (December 2).
Eisenberg recently told Digital Spy that he would "love" to film a sequel to Zombieland.
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Watch the red band trailer for Camp Hell below: