Social Network star Garfield, who takes over the iconic superhero role from Tobey Maguire, is seen wearing a Ramones t-shirt as he walks down an alleyway.
Additionally, a new image of Stone's Gwen Stacy has been released showing her character sheltering under an umbrella at a funeral.
(500) Days of Summer director Marc Webb's franchise reboot will explore Parker's time at high school and his romance with Gwen.
Rhys Ifans, Denis Leary, Martin Sheen, Sally Field and Irrfan Khan round out the cast for The Amazing Spider-Man, which will open in cinemas on July 3, 2012.
> The Amazing Spider-Man: Superhero reboot in pictures
> The Amazing Spider-Man: Andrew Garfield on-set pictures