Marc Webb has promised that his film is an "untold story" of Peter Parker.
"It's really important for us to be able to communicate that this isn't a remake of Sam Raimi's movie," he told Comic-Con Annual Magazine.
"There's a new territory, there's a new villain, it's a different Peter Parker."
Webb said that the film will be darker, but also incorporate some of Spider-Man's trademark wisecracks and banter.
"We wanted that humour to come from a real place," he said "My aim was to create a world where you could feel all those emotions.
"But are there moments of humour and levity and whimsy? Absolutely... There's a punk rock quality to Peter Parker that's really irreverent and fun and that's something that Andrew embodies in a way that we haven't seen before.
"Certainly the materials that have come out have a darker sentiment or there's a darker projection, but we're very keen on staying loyal to the humour of Spider-Man."
> Joss Whedon denies Spider-Man cameo in 'Avengers'
> 'The Amazing Spider-Man' to get sneak peek screenings
The Amazing Spider-Man stars Andrew Garfield, Emma Stone, Rhys Ifans, Denis Leary, Campbell Scott, Irrfan Khan, Martin Sheen and Sally Field.
The reboot will debut on July 3 in the US and on July 4 in the UK.
Watch Andrew Garfield talking to Digital Spy about The Amazing Spider-Man below: