The Marvel superhero movie's one-minute trailer was discussed over 33,000 times on Twitter in a study conducted by Way to Blue.
Starring Robert Downey Jr, Chris Evans and Mark Ruffalo, the blockbuster is being directed by Buffy the Vampire Slayer creator Joss Whedon.
The Battleship trailer, starring popstar Rihanna, was second in the list, while Andrew Stanton's sci-fi epic John Carter prompted the least reaction for its preview.
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Watch last night's Super Bowl trailer for The Avengers below: