The film is about a man, played by Adam Scott, who finds out he was part of a study into divorced children, and must deal with the chaos the present-day follow-up to the study causes in his family.
According to Deadline, Poehler will play the second wife to Richard Jenkins's character - whose ex-wife, played by Catherine O'Hara, is Poehler's enemy.
Ben Karlin and Stu Zicherman wrote the script for the movie, and Zicherman will also be directing. Filming begins this month in Atlanta.
This will be Amy Poehler and Adam Scott's first time acting together on the big screen. The two actors currently star on the hit NBC comedy Parks and Recreation.
Poehler just wrapped the fourth season of the show, for which she directed an episode set for broadcast in May. She also recently starred in a voice role for the animated film The Secret World of Arrietty.
Watch Amy Poehler in a clip from Parks and Recreation below: