The Andrew Stanton-directed fantasy, which stars Taylor Kitsch as an American Civil War veteran transported to Mars, has so far grossed $180 million at the worldwide box office. John Carter's rumoured production budget lies around the $250 million mark, but that doesn't factor in marketing costs to promote the film.
John Carter topped the UK box office last weekend, but has failed to gain traction in the US as it trails behind comedy 21 Jump Street and The Lorax.
The 3D film, based on the pulp novels from Tarzan creator Edgar Rice Burroughs, has also proved divisive with critics with a 51% rating on reviews aggregator Rotten Tomatoes.
In a statement released by Disney, the studio admitted that it is set to make a substantial loss on John Carter.
"In light of the theatrical performance of John Carter, we expect the film to generate an operating loss of approximately $200 million during our second fiscal quarter ending March 31," the company said.
"As a result, our current expectation is that the studio segment will have an operating loss of between $80 and $120 million for the second quarter."
Disney shares dropped 44 cents to $43 in extended trading on Monday, but the firm believes that upcoming movies The Avengers and Brave have "tremendous potential to drive value for the studio and the rest of the company".
> John Carter Lynn Collins interview
> John Carter Andrew Stanton interview
> Taylor Kitsch Twitter Q&A
Watch the trailer for John Carter below: