The Drive actor will appear opposite Elizabeth Olsen, Jessica Lange and Tom Felton in the adaptation of the 1867 novel by Émile Zola.
Isaac will appear as Laurent, the friend of Felton's character Camille, with whom she has an affair, reports Deadline.
The story follows Therese Raquin, who murders her selfish but well-meaning husband (Felton) with Laurent.
Elizabeth Olsen will play the title character in the project, to be directed by Charlie Stratton.
Isaac will also star as a villain in the upcoming Bourne Legacy opposite Jeremy Renner.
Watch Oscar Isaac in the trailer for The Bourne Legacy below: