The promo shows Andrew Garfield's web-slinging superhero patrolling New York at night, and also offers further glimpses of Rhys Ifans's villainous Lizard.
The trailer will debut in front of The Avengers in US cinemas, alongside the latest preview of The Dark Knight Rises.
Sony recently unveiled the first official stills of The Lizard, the alter-ego of Dr Curt Connors, played by Ifans.
A sequel to The Amazing Spider-Man is currently in development with Star Trek scribes Alex Kurtzman and Roberto Orci.
It has been reported that Spider-Man's origin story could span multiple films, with director Marc Webb describing the death of Peter Parker's parents as "an ongoing mystery".
Starring Garfield and Emma Stone, The Amazing Spider-Man will open on July 3 in the US and July 4 in the UK.
Photo gallery - The Amazing Spider-Man in pictures: