The Hot Fuzz actor returns as Montgomery 'Scotty' Scott in the sequel to the blockbuster 2009 film, also starring Zachary Quinto, Zoe Saldana and Chris Pine.
Benedict Cumberbatch has been cast as the villain in the JJ Abrams picture, with reports suggesting that he would be playing the fan favourite antagonist Khan, who made his first appearance in season one of Star Trek: The Original Series.
However, in an interview with The Daily Telegraph star Simon Pegg once again stated that Khan will play no part in the highly-anticipated film.
He said: "It's not Khan. That's a myth. Everyone's saying it is, but it's not. I think people just want to have a scoop. It annoys me - it's beyond the point to just ferret around for spoilers all the time to try to be the first to break them.
"It just spoils the film. It masquerades as interest in the movie but really it's just nosiness and impatience. You just want to say, 'Oh f**k off! Wait for the film!'"
A report in 2010 claimed that Khan would not feature in the film, with an insider alleging: "It's definitely a character that will make fans of (the original series) excited.
"Think along the lines of Harry Mudd or Trelane or Gary Mitchell or the Talosians or the Horta. Actually it's one of those that I named."
Star Trek 2 is due for release on May 17, 2013 in the US and the UK.
Watch Star Trek actor Anton Yelchin discuss the sequel with Digital Spy below: