Director Christopher Nolan's brother was responding to claims that the upcoming movie may suffer from having to accommodate a host of new characters such as Bane (Tom Hardy) and Catwoman (Anne Hathaway), as well as returning faces like Commissioner Gordon (Gary Oldman) and Batman himself (Christian Bale).
Noting previous comic book films Watchmen, Spider-Man 3, The League of Extraordinary Gentlemen and Iron Man 2, Sci Fi magazine asked Jonathan if he was worried the story would be too packed, to which he replied: "Oh, I don't believe in any of that s**t!
"Everybody is always looking for excuses as to why something worked and why something didn't work. Even The Dark Knight has three villains in it, or six, depending on how you're counting them.
"It is just a question of whether you can pull it off. That's what it comes down to. I think a narrative is only as cluttered as you let it be.
"There are as many good films with half a dozen villains as there are bad ones. And I think that is a sort of facile thing to seize on as an explanation for, 'This is why this worked, and this is why this didn't work'.
"Some of these things work because they're good, and some of these don't work because they're not. And I think if anyone had a formula for what makes one good, I would be very happy if they shared it with us, you know?"
Christopher previously revealed that he was initially nervous about including Catwoman in the film, but Jonathan convinced him the character could fit into the tone of the series.
The Dark Knight Rises, which has been rated 12A in the UK and PG-13 in the US, will be 164 minutes long, making it the longest Batman movie yet.
The Dark Knight Rises opens on July 20.
Watch Cillian Murphy discuss his rumoured appearance in The Dark Knight Rises with Digital Spy below: