Scenes involving Dr Curt Connors's (Rhys Ifans) family were cut, as was a "sexually unsettling" scene involving his alter ego The Lizard attacking and licking a teenage girl, Vulture reports.
Law & Order and Rubicon actress Annie Parisse was cast as Connors's wife Martha, but she was cut from the final film along with the character's son.
Another significant element that ended up being largely cut was the "first-person swinging", which featured heavily in early trailers.
A long take of Spider-Man (Andrew Garfield) swinging between New York skyscrapers was cut up into much shorter segments in the final film, and used only briefly - perhaps, Vulture speculates, to avoid a video-game like feel.
It has not yet been confirmed whether these scenes will be included on the film's DVD and Blu-Ray release.
The Amazing Spider-Man has broken Tuesday box office records in the US.
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Watch Digital Spy's UK premiere interviews with the cast of The Amazing Spider-Man below: