The short, which will feature on the Avengers Blu-ray/DVD, premiered at San Diego Comic-Con International over the weekend.
The clip features stars Lizzy Caplan and Jesse Bradford talking in a car moments before they step out to begin a heist.
The short centres around lovers Claire and Benny, who come into possession of an alien weapon following the invasion at the climax of The Avengers.
Meanwhile, SHIELD agents (Maximiliano Hernández and Titus Welliver) are hot on the modern-day Bonnie and Clyde's tails.
Item 47 continues the tradition of bonus shorts included in Marvel's DVD releases. Previous outings include the Agent Coulson-starring A Funny Thing Happened on the Way to Thor's Hammer and The Consultant.
Avengers will arrive on Blu-ray and DVD on September 17 in the UK and September 25 in the US.