The stop motion animation sees a young boy (Charlie Tahan) attempting to bring his beloved dog Sparky back to life via a risky science experiment.
Frankenweenie, which is a remake of Burton's 1984 short of the same name, features a voice cast including Winona Ryder, Martin Landau, Christopher Lee and Catherine O'Hara.
Its screening on the opening night of the 56th London Film Festival, October 10, will mark the film's European premiere.
"Frankenweenie is a perfect choice of opener – it's a film about the magic of movies from one of cinema's great visionaries.
"Tim Burton has chosen London as his home city and hundreds of talented British craftspeople have contributed to this production."
The full lineup for this year's BFI London Film Festival will be announced on September 5.
Last year's festival programme included Michel Hazanavicius's The Artist, Steve McQueen's Shame, David Cronenberg's A Dangerous Method, George Clooney's The Ides of March and Alexander Payne's The Descendants.
> 'Frankenweenie' gets new trailer at Comic Con 2012
> 'Frankenweenie' poster: Tim Burton returns to stop motion animation
Gallery - 'Frankenweenie' character portraits