Released by Superhero Hype, the spot focuses on Javier Bardem's villainous Raoul Silva, offering new footage of the character in addition to previously seen clips of Daniel Craig as Bond, Judi Dench as M, and the newly-cast Ben Whishaw as Q.
"How much do you know about fear?" Bérénice Marlohe's character Sévérine asks Bond, before explaining that he doesn't understand the extent of the threat Silva poses.
Director Sam Mendes has described the character of Silva as "full of surprises".
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Skyfall will be released on October 26 in the UK and November 9 in the US. Watch a trailer below: