Monday's Twilight after-party at LA Live included several wolves in cages located around the club's premises, in a reference to werewolves in the franchise.
(Pictured above: An image from 'The Twilight Saga')
PETA has now released a press statement slamming the party organizers for using live animals to celebrate the opening of a movie.
"Didn't it dawn on the Twilight event organizers that real wolves do not belong at a party with blaring music and flashing lights?" a PETA spokesperson wrote in their media release.
The statement continued: "The wolves were provided by Hollywood Animals, a notorious exhibitor that has been cited for numerous violations of the Animal Welfare Act for repeatedly failing to provide the animals it exploits with basic veterinary care, proper shelter, food, and water.
"We hope the rest of the promotional events surrounding Breaking Dawn celebrates the brilliant work of the cast and crew and shows enough respect for wildlife to leave animals out of it."
PETA's condemnation of the Breaking Dawn premiere party comes just days after the organisation unveiled a new campaign featuring the franchise's Christian Serratos.
Serratos poses in the adverts in a graveyard under the slogan 'Respect Life: Go Vegan'.
The Twilight Saga's Kellan Lutz also has expressed support for the animal rights group in the past.
Watch a trailer for The Twilight Saga: Breaking Dawn – Part 2 below:
Twilight: Breaking Dawn Part 2 world premiere: