The DreamWorks animated fantasy follows a carefree young boy (Chris Pine), who is enlisted by a team of Guardians - including Santa Claus (Alec Baldwin), the Tooth Fairy (Isla Fisher) and the Easter Bunny (Hugh Jackman) - to help protect the Earth.
The threat comes from the Nightmare King (Jude Law), who has hatched a plan to engulf the world in darkness.
In the clip, Pine's Jack Frost meets the Guardians after being transported via a magic portal, and is told that his ability to spread winter magic is needed in the fight against evil.
The film is directed by first-time feature helmer Peter Ramsey and executive produced by Guillermo del Toro,
> Hugh Jackman: 'Kids will love Rise of the Guardians'
> 'Rise of the Guardians': New Dreamworks animation unveils poster
Rise of the Guardians will be released on November 21 in the US, and November 30 in the UK.
Watch the trailer below: