The Smallville star has revealed that he has read for the part of Star-Lord.
"Read for my pal @JamesGunn Guardians of the Galaxy for Peter Quill. What a treat!" he wrote on Twitter.
The role of Peter Quill - the half-human, half-alien intergalactic superhero - has been linked to numerous actors in recent months.
Joseph Gordon-Levitt is the most recent name to be rumoured for the part in the Marvel Studios film. Lee Pace, Zachary Levi, Garrett Hedlund, James Marsden and Sullivan Stapleton are also said to have auditioned.
Rosenbaum is best known for playing iconic comic book villain Lex Luthor in the long-running Smallville television show.
Guardians of the Galaxy is scheduled for release on August 1, 2014.