Hugh Jackman and Anne Hathaway are among the cast members who will take part, having previously performed together at the 2009 ceremony which was hosted by Jackman.
The Daily Mail reports that Eddie Redmayne, Amanda Seyfried and Samantha Barks will also perform, although no song choices have yet been announced.
It is also unconfirmed whether the performance will comprise a single song, or a medley of numbers from the beloved Broadway show.
Photo gallery - Oscar nominees in pictures:will also feature a celebration of the James Bond franchise, which had its 50th anniversary last year.
Seth MacFarlane recently discussed his feelings about hosting the ceremony, joking that he feels no pressure because he expects to be "thrashed" by the press regardless of how he performs.
Watch Digital Spy discussing Les Misérables with Amanda Seyfried below: