Performers will feature the cast from Les Misérables including Hugh Jackman, Anne Hathaway, Russell Crowe, Amanda Seyfried, Eddie Redmayne, Samantha Barks and Helena Bonham Carter.
The celebration of musicals will include highlights from Chicago, Dreamgirls and Les Misérables.
Catherine Zeta-Jones and Jennifer Hudson will also be performing.
Show producers Craig Zadan and Neil Meron said: "We are pleased to have been able to amass so much talent to create the celebration of musicals of the last decade that we envisioned."
"We are thrilled that so many talented actors have agreed to bring our vision to life."
The 85th Annual Academy Awards will also see Adele singing Skyfall publicly for the first time.
Dame Shirley Bassey will be making her Oscars debut in a rumoured tribute to James Bond.
The Oscars will air on Sunday, February 24.
Oscars 2013 - Nominations in pictures: