The artwork shows Zach Galifianakis's Alan face off with Ken Jeong's Mr Chow in front of a burning Las Vegas backdrop, accompanied by the tagline: "It all ends."
The teaser image bears more than a passing resemblance to the first official poster for Harry Potter and the Deathly Hallows: Part 2.
The third film in the hugely-successful series will see the trio on a road trip, with no wedding or bachelor party, and will be very different from the second film, according to writer Craig Mazin.
Director and producer Todd Phillips has also revealed that the movie will revolve around the death of a character.
Galifianakis, Bradley Cooper, Ed Helms and Justin Bartha will all return, along with supporting cast members Jeffrey Tambor and Heather Graham.
The Hangover Part III will be released on May 24.
Watch Bradley Cooper discussing The Hangover Part II with Digital Spy below: