Adewale Akinnuoye-Agbaje, Rachel Nichols and Said Taghmaoui will star in the film based on the action figures and comic book series.
Sienna Miller and Ray Park have already agreed to appear in G.I. Joe, which stands for Global Integrated Joint Operating Entity.
Miller will play a baroness who is known for her spying skills, while Akinnuoye-Agjabe's role will be ordinance expert Heavy Duty.
Nichols has signed up to star as Scarlett, a skilled martial artist who specialises in hand-to-hand combat, while Taghmaoui is the team's communications specialist, Breaker.
The film, which will be set in Brussels, was written by Stuart Beattie. Stephen Sommers is directing the movie which is due for release next August.