Music
Rex Features
Bassey was rushed from her Monaco home to hospital last month after suffering from abdominal pains.
The 71-year-old had hoped to recover in time to perform at Mandela's Hyde Park gig, but was instructed to rest by doctors.
Her spokesman Paul Carey said: "The Dame is hugely disappointed and was looking forward to the show immensely."
He added: "On the advice of her surgeon, Dame Shirley Bassey will now not be able to perform as scheduled at the Nelson Mandela 46664 concert.
"Dame Shirley has been advised not to travel at the present time, so as to aid her continued full recovery following surgery last month."
ITV1 will air the concert "as live" on June 27.
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