The show, which will be hosted by Saturday Night Live comedian Andy Samberg, will be held at the Gibson Amphitheatre in Universal City, California, on May 31.
Eminem's performance will come just a few days after he releases his highly-anticipated sixth studio album Relapse.
Voting for the 18th annual awards began yesterday, with nominees being announced on May 4. Viewers will then be given another chance to vote for their favourites until May 18, although the best movie category will remain open throughout the ceremony.
Additional performers are scheduled to be announced nearer the time. Previous artists to have taken to the stage at the event include Coldplay, Foo Fighters, Christina Aguilera and the Pussycat Dolls.