The current UK Music chief executive spoke out in favour of the club, which is reportedly at risk from plans to develop a residential building opposite its flagship SE1 venue.
Sharkey said: "Ministry of Sound is a London landmark, a UK success story and a globally recognised brand. Any threat to such a core part of its business should be considered very carefully."
The superclub has also launched a petition expressing its opposition to the comments from property developer Oakmayne's chairman Christopher Allen that "nightclubs come and go"
The petition reads: "Ministry of Sound is not just a nightclub. Millions of people have passed through our doors over the 18 years we have been open.
"In the past we have and will always strive to create the moments people live for, and that people never forget."