'Telephone', featuring Beyoncé, held onto the number one spot after it emerged earlier this week that GaGa has clocked up more than a billion hits on YouTube.
Elsewhere in the chart, Boyzone's 'Gave It All Away' jumped 19 places to reach number ten, while Young Money's 'Bedrock' cracked the top ten for the first time, climbing three places to number nine. It first entered the charts at number 61 ten weeks ago.
Cheryl Cole's 'Parachute' climbed a place to reach number five, but Florence and Dizzee dropped a slot to number 11 with 'You've Got The Dirtee Love'.
Alicia Keys also fell out of the top ten, slipping five places to number 14 with 'Empire State Of Mind Part II'.
Meanwhile, Usher's 'OMG' featuring will.i.am entered the charts at number 13.
The top ten singles in full (click for our reviews):
1. (1) Lady GaGa ft. Beyoncé: 'Telephone'
2. (2) Tinie Tempah: 'Pass Out'
3. (4) Justin Bieber ft. Ludacris: 'Baby'
4. (3) Rihanna: 'Rude Boy'
5. (6) Cheryl Cole: 'Parachute'
6. (8) INNA: 'Hot'
7. (5) Ellie Goulding: 'Starry Eyed'
8. (7) Jason Derulo: 'In My Head'
9. (12) Young Money ft. Lloyd: 'Bedrock'
10. (29) Boyzone: 'Gave It All Away'
Source: Official Charts Company
> Click here for this week's top ten albums