The 'Jeremy' band will take the stage on October 23 and October 24 in Northern California for an annual show put together by Neil Young.
The event will raise funds for the Bridge School, an institution that helps children with physical impairments.
Guitarist Stone Gossard told Reuters that the benefit concert will be the perfect place to celebrate the band's platinum anniversary because of the tremendous admiration they have for Young.
"The only thing we've got going on in October is Bridge School, which is really special," he said. "So that's our 20th anniversary, which is fitting. It's perfect, not too blown-up. It's not about us, it's about all those kids that are on stage with you and about Neil Young and his commitment, his influence."
The musician added that playing together for two decades is an important milestone.
"It's so great that it's still going on, isn't it? 'Just stick together', that's the advice we got from [U2's manager] Paul McGuinness.
"I think we've just stayed on course in a way that's maybe encouraged people and surprised people, and I think there's more that we can do."
Pearl Jam recently finished a worldwide tour to support their latest album Backspacer.