The Official Chart Company has compiled the list of duets for a BBC Radio 2 countdown later today from DJ Tony Blackburn.
The duo also came second in the chart, with another Grease number: 'Summer Nights'. Puff Daddy and Faith Evans' tribute to Notorious B.I.G., 'I'll Be Missing You', came third.
Jason Donovan and Kylie Minogue's 'Especially for You' finished fourth, while Elton John's duet with Kiki Dee on 'Don't Go Breaking My Heart' completed the top five.
Chart analysts ruled that songs would only be counted if duos received equal billing on the track, while guest vocalists or groups would not be included.
The top ten in full:
1. John Travolta and Olivia Newton-John - 'You're The One That I Want'
2. John Travolta and Olivia Newton-John - 'Summer Nights'
3. Puff Daddy and Faith Evans - 'I'll Be Missing You'
4. Jason Donovan and Kylie Minogue - 'Especially For You'
5. Elton John and Kiki Dee - 'Don't Go Breaking My Heart'
6. Windsor Davies and Don Estelle - 'Whispering Grass'
7. Elaine Paige and Barbara Dickson - 'I Know Him So Well'
8. Paul McCartney and Stevie Wonder - 'Ebony and Ivory'
9. David Bowie and Mick Jagger - 'Dancing In The Street'
10. Phil Collins and Philip Bailey - 'Easy Lover'