The track, which was crowned Billboard's 1,000th chart topper last week, clocks up a second week at the summit, fending off competition from Cee Lo Green's 'Forget You', which launches up the chart from seven to two.
Elsewhere in the top ten, Dr Dre, Eminem and Skylar Grey's 'I Need A Doctor' vaults 32 to four, Rihanna's 'S&M' climbs from eight to seven, and Justin Bieber and Jaden Smith's 'Never Say Never' leaps from 25 to eight.
Further down the chart, Usher reaches a new peak on the tally with 'More', which advances 21 to 15 after 11 weeks on the chart, while Adele's 'Rolling In The Deep', jumps from 41 to 26.
Meanwhile, Katy Perry's 'E.T' re-enters at number 28, and Tinie Tempah lands at 91 with 'Written In The Stars'.
The top ten singles in full (click where possible for our reviews):
1. (1) Lady GaGa: 'Born This Way'
2. (7) Cee Lo Green: 'F**k You'
3. (3) Bruno Mars: 'Grenade'
4. (32) Dr Dre feat. Eminem, Skylar Grey: 'I Need A Doctor'
5. (2) Katy Perry: 'Firework'
6. (5) Pink: 'F**kin' Perfect'
7. (31) Rihanna: 'S&M'
8. (25) Justin Bieber feat. Jaden Smith: 'Never Say Never'
9. (6) Enrique Iglesias ft. Ludacris & DJ Frank E: 'Tonight (I'm Lovin' You)'
10. (4) Wiz Khalifa: 'Black And Yellow'