The children's TV character joins the Gruffalo and the Zingzillas on the Kidz Field during the festival at Worthy Farm, Pilton from June 22 to June 26.
"Another superstar of the children's books/TV world has been added to the bill," organisers confirmed.
"Rastamouse, the reggae-playing, crime-fighting mouse who's become something of a phenomenon since hitting TV screens at the beginning of this year, will make his worldwide live debut at this year's festival, with a daily performance alongside his Easy Crew."
The BBC recently responded to criticisms of Rastamouse after some viewers claimed that "the use of non-standard English grammar in the programme sets a bad example to young children".
Beady Eye frontman Liam Gallagher more recently confirmed that he is a fan of the series.
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Watch the Rastamouse 'Ice Popp' video below: