The rehearsal clip, which shows the singer preparing for her 26-date jaunt across the US, appeared on YouTube yesterday.
The one minute preview sees the star performing the track 'How I Roll', which features on her latest album Femme Fatale, in a tracksuit and surrounded by dancers.
Spears's tour kicks off in Sacramento, California on June 16.
It was recently confirmed that her longtime choreographer Brian Friedman had pulled out of the project due to "scheduling conflicts".
Watch the rehearsal footage below: