The promo for the track, taken from their fifth studio album Come Around Sundown, was shot in the band's home state of Tennessee.
The clip sees the group perform an outdoor show in the local town, while various residents make their way to the concert.
Kings of Leon member Matthew Followill became a father last month after his wife gave birth to a baby boy, while his cousin Caleb Followill recently married Victoria's Secret model Lily Aldridge.
The 'Use Somebody' band will play two Hyde Park shows later this month with special guest Paul Weller.
Watch the video for Kings of Leon's 'Back Down South' below: