The 29-year-old performer will kick off the shows in Sacramento, California on June 16. Following a summer touring the US, the pop icon will visit the UK and Ireland in October.
'Femme Fatale's setlist features a mixture of old and new hits from Spears, including recent chart entries 'Hold It Against Me' and 'Till The World Ends', as well as former chart-toppers 'Baby One More Time' and 'Toxic'.
Spears's cover of Madonna's 'Burning Up' has also made the cut, along with a solo rendition of Rihanna's 'S&M', which the popstar recently featured on a remix of.
Fan-favourite 'I'm A Slave 4 U' features, but Spears's number one ballad 'Everytime' has not been included as part of the 21-track setlist.
> Britney Spears explores 'Femme Fatale' tour stage: Video
The 'Femme Fatale' tour setlist is as follows:
1. 'Hold It Against Me'
2. 'Up N' Down'
4. 'Piece of Me'
5. 'Big Fat Bass'
6. 'How I Roll'
7. 'Lace and Leather'
8. 'If U Seek Amy'
9. 'Gimme More'
10. '(Drop Dead) Beautiful (feat. Sabi)'
11. 'He About To Lose Me'
12. 'Boys (Remix)'
13. 'Don't Let Me Be The Last To Know'
14. 'Baby One More Time'
16. 'Trouble For Me'
17. 'I'm A Slave 4 U'
18. 'Burning Up (Madonna cover)'
19. 'I Wanna Go'
22. 'Till The World Ends'
Watch footage of Britney Spears rehearsing for the show below: