The track, which has spent a total of 16 weeks on the tally, continues to fend off Pitbull's 'Give Me Everything', which remains at number two, while Adele's 'Someone Like You' completes the top three.
Elsewhere in the Top 10, Stan Walker's 'Loud' advances from 18 to nine, and Jessie J moves from 11 to ten with 'Nobody's Perfect'.
Further down the chart, Christina Perri vaults from 23 to 12 with 'Jar of Hearts', while Bruno Mars leaps from 38 to 25 with 'Marry You'.
S. mouse, from comedy show Angry Boys, debuts at 37 with 'Slap My Elbow', and Aloe Blacc's 'I Need A Dollar' lands at 50.
The Top 10 singles in full (click where possible for our reviews):
1. (1) LMFAO ft. Lauren Bennet & GoonRock: 'Party Rock Anthem'
2. (2) Pitbull ft. Ne-Yo, Afrojack & Nayer: 'Give Me Everything'
3. (4) Adele: 'Someone Like You'
4. (3) Adele: 'Rolling In The Deep'
5. (5) Jason Derulo: 'Don't Wanna Go Home'
6. (6) Katy Perry: 'Last Friday Night'
7. (7) DJ Havana Brown: 'We Run The Night'
8. (8) Marvin Preist: 'OwnThis Club'
9. (18) Stan Walker: 'Loud'
10. (11) Jessie J: 'Nobody's Perfect'
Watch LMFAO's 'Party Rock Anthem' music video below: